On Monday 22 September at 13:00, Louise Bidstrup-Bendixen defends her PhD dissertation entitled "Exploring the possibilities and limitations of CRISPR/Cas-based transcriptional modulation".

The CRISPR gene editing system enables both temporary and permanent genomic alterations. One application of the CRISPR system is the modulation of gene expression, termed CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi). The development and optimisation of CRISPRa and CRISPRi systems is the focus of a new PhD project.

The project shows some of the many possibilities of CRISPR-based transcriptional modulation by exploring new CRISPRa and CRISPRi constructs, multiplexing, and introducing a CRISPR system that allows for simultaneous activation, interference, and knockout of three separate genes. The project also explores limitations of CRISPRa and long-term gene activation by CRISPR-based epigenome editors by analysing the epigenetic background of target genes and the effect of the CRISPR systems across cell types.

The summary is written by the PhD student.

The defence is public and takes place in auditorium 1252-310 (Jeppe Vontilius), Aarhus University. Please see the press release for more information. 

Contact

PhD student Louise Bidstrup-Bendixen
Mail: lbx@biomed.au.dk  
Phone: 22480522

Read full press release